2000L Fermentor

Henrik  Grothe

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Raps er en biomasse der kan anvendes i fermentering og raffinering for skabe biosolutions produkter af høj værdi og som er et mere bæredygtigt alternativ

2000L Fermentor

A 2000L fermentor is used to carry out biofermentation processes on a pilot scale. Testing at this scale typically follows testing at 200L. At the Danish Technological Institute, we are in the process of establishing a facility for the installation of our 2000L fermentor. Contact us for more information if there is a need for testing at this scale.

A fermentation process is used, among other things, for the development of food, feed, or food ingredients (e.g. proteins). The biomass, which can consist of microorganisms or plant material, undergoes a transformation using, for example, yeast or bacteria. 

In a biofermentation process, biotechnology is used to select, modify, and optimize microorganisms in order to produce specific biosolutions. In biofermentation processes, the role of biotechnology is to exploit microorganisms such as bacteria, yeast, fungi, or plant cells for the production of desired biosolutions. 

The biofermentation process has the potential to produce a wide range of biosolutions for industries such as food production, biochemical production, and pharmaceutical production.

The available gases we work with

• Air

• O2

• N2

• CO2

• H2.

We can cultivate aerobically and anaerobically with the following microorganisms

• Fungi

• Bacteria

• Microalgae

• Yeast

Our 2000L fermentor

Gas supply controlled by MFC; ring sparger; gas mixing chamber.

Variable speed, temperature, pH, and gas supply for optimization.

Sensors: Temperature, pH, DO, foam.

Option to add batch with two different feed media.

  • 500 - 2200 L working volume
  • 40 – 250 rpm rotation speed
  • 5 – 135 oC working temperature
  • Air supply 2 VVM, additional gas supply (CO2, N2, O2, H2) 0.5 VVM

RF2000.1 - stainless steel fermentor (2915 L Total volume):

Working volume500-2200 L
MFC for Air flow rate (l/min)44 - 2200 L/min (1 VVM)
Co2 flow rate / o2 flow rate / n2 flowrate / H2 flowrate0.3 - 0.5 (VVM) / 0.3 - 0.5 (VVM) / 0.3 – 0.5 (VVM) / 0.3 – 0.5 (VVM)
Mixer typeBottom mounted 3x Rushton
Mixer rotation speed (rpm)40-250 (rpm)
Vessel Operating temperature (°C)5-135 °C

How can Danish Technological Institute help you?

The 2000L fermentor, which we are in the process of establishing a facility for the installation, is a part of Danish Technological Institute's Biosolutions Technology Center, where we offer laboratory and pilot testing in the field of biorefining and biofermentation. Our equipment is often involved as a subprocess in the development of innovative products - either in research and development projects or in direct collaborations with companies.

Offer and prices

If you are interested, you can contact Henrik Grothe