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Sequencing for identification of bacteria

Lene  Meinert

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Sequencing for identification of bacteria

Molecular biological methods allow us to estimate the microflora in a completely new way as the methods provide a complete picture of the composition of the present flora in e.g. a production environment. It is also possible to present a very accurate identification of e.g. Listeria by sequencing the genome of the entire bacterium.

Relative distribution of bacteria in e.g. a production environment

Analysis of the relative bacterial distribution in a complex sample is possible by sequencing the 16S rRNA gene. When using this analysis, changes in production and contamination paths/places in the production can be identified by looking at changes in the bacterial flora.

We offer

  • DNA extraction
  • Quality control
  • Preparation of an amplicon sequencing library (16S V3-V4 region)
  • Illumina MiSeq sequencing (2 x 300 bp)
  • OTU clustering estimates for the relative distribution and taxonomic classification


Exact identification of pathogenic bacteria

Analysis of bacterial pathogens for source tracing is possible by using whole genome sequencing (WGS). By this analysis, pathogens can be identified at DNA level, and clones of the same bacterial species can be compared and differentiated.

We offer

  • DNA extraction
  • Quality control
  • Preparation of an amplicon sequencing library
  • Illumina MiSeq sequencing (2 x 300 bp)
  • Relevant bioinformatics – e.g. taxonomic classification, typing and snp analysis

We offer to perform an inspection of the production, relevant sampling, analysis and company-specific consultancy on the use of results and to point out possible corrective actions in the production.

 

Please contact us for information on prices then we will tailor-make an offer for you.